Aromatic Peroxygenase Structures

January 9, 2014

Tweet Aromatic peroxygenases have catalytic activity similar to that of cytochrome P450 monoxygenases (hydroxylation, S-oxidation, N-oxidation) on both aromatic and aliphatic substrates.  Unlike P450 monooxygenases, these enzymes have no need for NAD(P)H and complicated reductase domains and instead use hydrogen peroxide as the oxidant. The aromatic peroxygenase from the edible commercial mushroom, Agrocybe aegerita, is one of the well-studied enzymes […]

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Better Biocatalyst Reaction Schemes

December 20, 2013

Tweet Biocatalysts are increasingly being used by synthetic chemists.  For some reactions, such as stereoselective ketone reduction or resolution of carboxylic acids and esters, ketoreductases (KREDs) and lipases are well known and a first-line option.  However, there are a wide variety of enzymes that do useful chemistry that are not routinely used within the organic […]

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Omega Transaminase Review

December 19, 2013

Tweet Kroutil and co-workers have published a review on cascade reactions involving omega transaminases.  Cascade strategies are a powerful way to drive the equilibria of transaminase reactions, and the reductive amination of a substrate is often driven by concurrent enzymatic consumption of a co-product.  Many other cascades, for example the oxidation of an alcohol to a carbonyl followed by transaminase-mediated reduction […]

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John Cornforth and Enzyme Stereoselectivity

December 16, 2013

Tweet Sir John Cornforth passed away on December 14th, 2013.  Cornforth was a pioneer in the elucidation of the stereochemistry of enzyme reactions.  A chemist by training, Australian-born Cornforth contributed to the synthesis of steroids while conducting doctoral studies with Sir Robert Robinson at Oxford.  Cornforth’s independent research involved labeling enzyme substrates with deuterium and tritium using synthetic chemistry, generating isotopically-chiral […]

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Let’s Meet at Informex in Miami

December 12, 2013

Tweet Just a quick note to let everyone know that David Rozzell will be at Informex in January. If you would like to schedule a meeting, contact me by email: And we have a special offer on the Enzyme Sources Guide through the end of December: All purchasers will get a free updated 2014 […]

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New Mainstream Biocatalysis Newsletter: Profiles on Verenium, Toluene Dioxygenase

December 3, 2013

Tweet The latest Mainstream Biocatalysis Newsletter came out this morning. In this issue we cover the latest company news, publications, and published patent applications. There are also two new profiles. The Company Profile is Verenium Corporation, which is timely since Verenium was recently acquired by BASF. The Enzyme Profile is toluene dioxygenase, an enzyme first […]

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Biocatalytic Retrosynthesis

November 4, 2013

Tweet E. J. Corey pioneered retrosynthetic analysis in organic synthesis, In fact, I was fortunate enough to take his course covering the subject in graduate school, and it was tour-de-force for synthetic organic chemists. Now, with biocatalysis surging and rightly staking a claim to be a viable option for chemical production, couldn’t the Corey concepts […]

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Intelligent Enzyme Intelligence: Enzyme Sources Guide

November 4, 2013

Tweet Ryan and I have just created the Enzyme Sources Guide, a publication we hope will be an indispensable resource covering the enzyme industry. With 461 pages containing profiles of more than 200 enzyme producers, developers, and suppliers around the world, the Enzyme Sources Guide is the most comprehensive compendium available covering the players in the enzyme […]

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Vitamins for Ethanol?

October 31, 2013

Tweet Calling it a “vitamin shot” for ethanol production, exactly one year ago Novozymes launched Avantec, a new enzyme product that raised ethanol yields from corn. The enzyme raises the amount of ethanol produced from corn starch by 2.5%. That may not sound like much, but with more than 13 billion gallons of ethanol produced, that […]

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Recent Biocatalysis Publications and Patents, Halogenases, and More

October 22, 2013

Tweet The latest Mainstream Biocatalysis Newsletter went out this morning. In addition to the latest publications and patent applications, this issue features halogenases in the enzyme profile section and Finnish enzyme company Metgen Oy in the company profile.  Wnat to know more? Sign up in the sign-up box to the right. To view the most […]

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